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Anti- Active Rac1 Mouse Monoclonal Antibody

價格￥4800.00
品牌NewEast Biosciences   
產地中國 武漢
貨號26903
規格30μl
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    Active Rac1 GTP [26903]                                                                                                                                             

    [30 µL]
    Catalog Number:  26903
    Gene Symbol:  RAC1
    Description:   Anti Active Rac1-GTP Mouse Monoclonal Antibody
    Background:   Small GTPases are a super-family of cellular signaling regulators. Rac belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Rac, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).
    Immunogen:   Recombinant full length protein of active Rac1
    Applications:   IP, IHC
    Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP; 
                                                       1:100 dilution for IHC
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgM
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti-active Rac1 antibody recognizes active Rac1 from vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
      
    Immunoprecipitation/Western blot:  
    Rac activation assay. MEF cells were treated with (lane 2) or without (lane 1) PDGF. Cell lysates were incubated with an anti active Rac monoclonal antibody (Cat # 26903) (top panel). Th precipitated active Rac was immunoblotted with an anti Rac rabbit polyclonal antibody (Cat # 21003). The bottom panel shows the Western blot with anti Rac of the cell lysates used (5% of that used in the top panel).
    Immunofluorescence:  
    Immunofluorescence for the active Rac using Anti Active Rac1 GTP Mouse Monoclonal Antibody [26903] shows Rac GTP immunolabeling (green) in combination with cofilin (red) on brain tissue sections. 
    The tissue sections were fixed with -20 ºC methanol or 4% paraformaldehyde (fixation time 1hr) and stained with antibody at 1:1000 in 0.1M Phosphate buffer with 0.3% Triton X, and 4% BSA for 24h at room temperature. Secondary antibodies were anti mouse AlexaFluor488 and anti rabbit AlexaFluor594 at 1:1000.

    Related Products

     

    Catalog# Name Size Price
    80501 Rac1 pulldown  Activation Assay Kit  30 assays  ¥6800.00
    26903 Active Rac1-GTP Monoclonal Antibody  30 μL   ¥4800.00
    26005 Anti Rac1 Mouse Monoclonal Antibody  100 μL   ¥2800.00
    21003 Anti Rac1 Rabbit Polyclonal Antibody  100 μL   ¥2600.00
    21027 Anti Rac2 Rabbit Polyclonal Antibody  100 μL   ¥2600.00

     

     

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