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Anti- CTNNB1(S33F) Mouse Monoclonal Antibody

價格¥4800.00
品牌NewEast Biosciences   
產地中國 武漢
貨號26304
規格100ul
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  • CTNNB1(S45P)_26307

    [100ul]

     

    Catalog Number:  26307
    Gene Symbol:  Beta catenin, CTNNB, Catenin beta-1
    Description:   Anti CTNNB1(S45P) Mouse Monoclonal Antibody
    Background:   CTNNB1 protein is a dual function protein. It is a subunit of a complex of proteins that from adherens junctions, which are important for the establishment and maintenance of epithelial cell layers by regulating cell growth and adhesion between adjacent cells. CTNNB1 protein also pulls double duty as an intracellular signal transducer in the Wnt signaling pathway. Mutations of CTNNB1 have been implicated in the pathogenesis of several cancers.
    Immunogen:   A synthetic peptide from the internal region of CTNNB1 which includes the mutation of S45P, human origin.
    Applications:   ELISA, IF, IHC
    Recommended Dilutions:   
    ELISA: 1:1000-1:2000 
    IF: 1:50-1:100 
    IHC: 1:50-1:100
    Concentration:   0.5 mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes S45P mutant, but not wild-type CTNNB1 of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
     
    Immunofluorescence::  
    Immunofluorescence of cells expressing CTNNB1 proteins with anti CTNNB1(S45P) antibody. HEK293T cells were transfected with pCDNA3-GFP CTNNB1(S45P) plasmid, pCDNA3-GFP CTNNB1(WT) plasmid pCDNA3-GFP CTNNB1(S45F) plasmid or pCDNA3-GFP CTNNB1(S45Y) plasmid, then fixed and stained with anti CTNNB1(S45P) monoclonal antibody (Cat. #26307).


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