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neweastbio@126.com
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| 價格 | ￥4800.00 |
| 品牌 | NewEast Biosciences  |
| 產地 | 中國 武漢 |
| 貨號 | 26072 |
| 規格 | 100ul |
| 說明書 | 說明書下載 |
TP53(R175H) mono [26072]
[100 µL]
| Catalog Number: 26072 |
| Gene Symbol: TP53(R175H) |
| Description: Anti TP53(R175H) Mouse Monoclonal Antibody |
| Background: TP53 belongs to the p53 proteins family. It is ubiquitously expressed, acts as a tumor suppressor in many tumor types and induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Defects in TP53 are a cause of esophageal cancer (ESCR), lung cancer (LNCR), adrenocortical carcinoma (ADCC), choroid plexus papilloma (CPLPA) and so on. |
| Immunogen: A synthetic peptide from the internal region of TP53 which includes the mutation of R175H, human origin |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:1000-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti TP53(R175H) antibody recognizes TP53(R175H) of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles. |
| Western blot: |
 |
Western blot analysis of recombinant TP53(R175H) and wildtype proteins. Purified His-tagged TP53(R175H) protein(Cat.#10207) and wildtype protein(Cat.#10202) were blotted with Anti TP53(R175H) monoclonal antibody(Cat.# 26072). |
| Immunofluorescence: |
|
Immunofluorescence of cells expressing TP53 proteins with anti TP53(R175H) antibody. HEK293T cells were transfected with pCDNA3-GFP TP53(WT) plasmid (left column) or pCDNA3-GFP TP53(R175H) plasmid (right column), then fixed and stained with anti- TP53(R175H) monoclonal antibody (Cat. # 26072). |
| Related Products | |||
| Catalog# | Name | Size | Price |
| 21270 | Anti TP53 Rabbit Polyclonal Antibody | 100 μL | ￥2,800 |
| 26076 | Anti TP53(R282W) Mouse Monoclonal Antibody | 100 μL | ￥4800 |
| 26072 | Anti TP53(R175H) Mouse Monoclonal Antibody | 100 μL | ￥4800 |
| 21165 | Anti TP53(R282W) Rabbit Polyclonal Antibody | 100 μL | ￥4800 |
| 21161 | Anti TP53(R175H) Rabbit Polyclonal Antibody | 100 μL | ￥4800 |
