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Anti -Active RhoA Mouse Monoclonal Antibody

價(jià)格￥4800.00
品牌NewEast Biosciences   
產(chǎn)地中國(guó) 武漢
貨號(hào)26904
規(guī)格30μl
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  • Active RhoA GTP [26904]
    [30 µL]

     

     

    Catalog Number:  26904
    Gene Symbol:   RHOA
    Description:   Anti Active RhoA Mouse Monoclonal Antibody
    Background:   Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca 2+ sensitivity of smooth muscle contraction.
    Immunogen:   Recombinant full length protein of active RhoA
    Applications:   IP, IHC
    Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP; 
                                                       1:100 dilution for IHC
    Concentration:   1 mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:  Monoclonal
    Isotype:   IgG2b
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti-active RhoA antibody recognizes active RhoA from vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
     
    Immunoprecipitation/Western Blot:  

    IP/WB analysis of active RhoA protein. Purified recombinant RhoA proteins wer
    e loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated
    with anti active RhoA mouse monoclonal antibody (Cat. # 26904). After SDS/PAGE, the
    membrane filter was probed with anti RhoA mouse monoclonal antibody (Cat. # 26007).

     

     

     

    Related Products
    Catalog# Name Size Price
    80601 RhoA pulldown Activation Assay Kit  30 assays  ￥6800  
    26904 Anti -Active RhoA Mouse Monoclonal Antibody  30 μL  ￥4800
    26007 Anti -RhoA Mouse Monoclonal Antibody  100 μL  ￥2800
    21009 Anti- RhoA Rabbit Polyclonal Antibody  100 μL  ￥2600

     

     

     

     

     

  • 示例結(jié)果

     

    下圖展示的是武漢紐斯特生物技術(shù)有限公司的 RhoA 活性試劑盒的典型結(jié)果。下面的數(shù)據(jù)僅供參考。 

     

     

     

     

    Rho 活性分析。小鼠胚胎成纖維細(xì)胞（MEF）用血小板衍生因子（PDGF）處理（Lane 2）和未處理（Lane 1）。細(xì)胞裂解物用特異性識(shí)別 RhoA 活性構(gòu)象的鼠單克隆抗體(Cat. # 26904)孵育(上圖)。親和沉淀物用anti-RhoA pAb(Cat # 21009)進(jìn)行免疫印跡實(shí)驗(yàn)。底部的圖展示的是細(xì)胞裂解液的活性 RhoA 的 Western blot 實(shí)驗(yàn)結(jié)果。

     

  • Predicted MW.   22 kDa

    Observed MW.   22 kDa

    Uniprot ID.       P61586

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