武漢紐斯特生物技術有限公司歡迎您！
neweastbio@126.com
neweastbio@126.com
| 027-87561033
PRODUCT CENTER
| 價格 | ￥4800.00 |
| 品牌 | NewEast Biosciences  |
| 產地 | 中國 武漢 |
| 貨號 | 26186 |
| 規格 | 100ul |
| 說明書 | 說明書下載 |
RAS(G12S) [26186]
[100ul]
| Catalog Number: 26186 |
| Gene Symbol: H-Ras; K-Ras; N-Ras |
| Description: Anti RAS(G12S) Mouse Monoclonal Antibody |
| Background: Ras protein is a membrane-associated GTPase signaling protein that regulates proliferation, differentiation, and cell survival. Ras G12S mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions. |
| Immunogen: A synthetic peptide from the internal region of RAS which includes the mutation of G12S, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes G12S mutant, but not wild-type RAS of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Western blot: |
 |
Western blot analysis of recombinant RAS(G12S) and wildtype proteins. Purified His-tagged RAS(G12S) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti RAS(G12S) monoclonal antibody (Cat. #26186). |
| Immunofluorescence: |
|
|
| Immunofluorescence of cells expressing RAS proteins with Anti RAS(G12S) antibody. HEK293T cells were transfected with pCDNA3-GFP Ras(G12S) plasmid, pCDNA3-GFP Ras(WT)plasmid,pCDNA3-GFP Ras(G12S) plasmid, pCDNA3-GFP Ras(G12V)plasmid,pCDNA3-GFP-Ras(G12C)plasmid, pCDNA3-GFP Ras(G12R)plasmid or pCDNA3-GFP Ras(G12A)plasmid, then fixed and stained with Anti RAS(G12S) monoclonal antibody (Cat. #26186). |
| Related Products | |||
| Catalog# | Name | Size | Price |
| 26909 | Anti Active Ras Mouse Monoclonal Antibody | 30μl | ￥4,800 |
| 81101 | Ras Activation Assay Kit | 20T | ￥6,800 |
| 21021 | Anti Ras Rabbit Polyclonal Antibody | 100 μL | ￥2,600 |
| 26014 | Anti Ras Mouse Monoclonal Antibody | 100 μL | ￥2,800 |
| 26479 | Anti RAS(A146V) Mouse Monoclonal Antibody | 100 μL | ￥4,800 |
| 26468 | Anti RAS(K117N) Mouse Monoclonal Antibody | 100 μL | ￥4,800 |
| 26478 | Anti RAS(A146P) Mouse Monoclonal Antibody | 100 μL | ￥4,800 |
| 26477 | Anti RAS(G13V) Mouse Monoclonal Antibody | 100 μL | ￥4,800 |
| 26474 | Anti RAS(G13R) Mouse Monoclonal Antibody | 100 μL | ￥4,800 |
| 26192 | Anti Ras(Q61R) Mouse Monoclonal Antibody | 100 μL | ￥4,800 |
| 26193 | Anti Ras(Q61L) Mouse Monoclonal Antibody | 100 μL | ￥4,800 |
| 26195 | Anti Ras(Q61H) Mouse Monoclonal Antibody | 100 μL | ￥4,800 |
| 26191 | Anti HRAS(G13A) Mouse Monoclonal Antibody | 100 μL | ￥4,800 |
| 26186 | Anti RAS(G12S) Mouse Monoclonal Antibody | 100 μL | ￥4,800 |
| 26261 | Anti RAS(A146T) Mouse Monoclonal Antibody | 100 μL | ￥4,800 |
| 26038 | Anti Ras(G13D) Mouse Monoclonal Antibody | 100 μL | ￥4,800 |
| 26036 | Anti Ras(G12D) Mouse Monoclonal Antibody | 100 μL | ￥4,800 |
| Publications: |
| 1. DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model Gene Therapy 21, 888-896 (October 2014) |
| 2. Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a caecal adenocarcinoma and contiguous tubulovillous adenoma J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136 |
發表時間：2016年
名稱/貨號：Anti- RAS(G12S) Mouse Monoclonal Antibody /26186
影響因子：Mol Ther Nucleic Acids IF=8.8
應用范圍：WB
種屬：Human
發表時間：2017 年
名稱/貨號：
影響因子：Mol Ther Nucleic Acids IF=8.8
應用范圍：WB
種屬：Mouse
發表時間：2020年
名稱/貨號：Anti-RAS(G12S) Mouse Monoclonal Antibody /26186
影響因子：Theranostics IF-12.4
應用范圍：WB IHC
種屬：Human Mouse
