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neweastbio@126.com
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| 價格 | ￥4800.00 |
| 品牌 | NewEast Biosciences  |
| 產地 | 中國 武漢 |
| 貨號 | 26193 |
| 規格 | 100ul |
| 說明書 | 說明書下載 |
Ras(Q61L) [26193]
[100 µL]
| Catalog Number: 26193 |
| Gene Symbol: Ras(Q61L) |
| Description: Anti Ras(Q61L) Mouse Monoclonal Antibody |
| Background: Ras(Q61L) is one type of Ras missence mutations at codon 61,Ras protein is a membrane-associated GTPase signaling protein that regulates proliferation, differentiation,and cell survival. Ras Q61L mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions. |
| Immunogen: A synthetic peptide from the internal region of Ras which includes the mutation of Q61L, human origin. |
| Applications: ELISA, WB, IF, IHC |
| Recommended Dilutions: ELISA 1:100-1:5000 WB 1:100-1:1000 IF 1:50-1:100 IHC 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes Ras(Q61L), but not wild type Ras protein from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Western blot: |
 |
Western blot analysis of recombinant Ras proteins. Equal amount of purified Ras Q61H (lane 1), Ras Q61L (lane 2), RasQ61R (lane 3), kRas WT (lane 4), hRas WT (lane 5), then were loaded and blotted with anti-Ras Q61L mouse monoclonal antibody (Cat. #26193). |
| Immunofluorescence: |
|
| Immunofluorescence of cells expressing Ras proteins with Anti Ras(Q61L) antibody. HEK293T cells were transfected with pCDNA3-GFP-Ras(Q61L) plasmid, pCDNA3-GFP Ras(WT) plasmid, pCDNA3-GFP Ras(Q61H) plasmid, pCDNA3-GFP Ras(Q61R) plasmid or pCDNA3-GFP Ras(Q61K) plasmid, then fixed and stained with Anti Ras(Q61L) monoclonal antibody (Cat. #26193). |
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| Publications: |
| 1. DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model Gene Therapy 21, 888-896 (October 2014) |
| 2. Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a caecal adenocarcinoma and contiguous tubulovillous adenoma J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136 |
