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neweastbio@126.com
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| 價格 | ￥4800.00 |
| 品牌 | NewEast Biosciences  |
| 產地 | 中國 武漢 |
| 貨號 | 26253 |
| 規格 | 100ul |
| 說明書 | 說明書下載 |
FGFR1(P252T)
[100ul]
| Catalog Number: 26253 |
| Gene Symbol: FGFR1,BFGFR,CD331,CEK,FGFBR,FLG |
| Description: Anti FGFR1(P252T) Mouse Monoclonal Antibody |
| Background: The protein FGFR1 is highly conserved between members and throughout evolution. This particular family member of FGFR1 binds both acidic and basic fibroblast growth factors and is involved in limb induction. Mutations of FGFR1 protein have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, squamous cell lung cancer and autosomal dominant Kallmann syndrome. |
| Immunogen: A synthetic peptide from the internal region of FGFR1 which includes the mutation of P252T, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:2000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes P252T mutant, but not wild-type FGFR1 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Western blot: |
 |
Western blot analysis of recombinant FGFR1(P252T) and wildtype proteins. Purified His-tagged FGFR1(P252T) protein (22-376aa, ~45KDa, lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti FGFR1(P252T) monoclonal antibody (Cat. #26253). |
| Immunofluorescence: |
|
Immunofluorescence of cells expressing FGFR1 proteins with Anti FGFR1(P252T) antibody. HEK293T cells were transfected with pCDNA3-GFP FGFR1(WT) plasmid (left column) or pCDNA3-GFP-FGFR1(P252T) plasmid (right column), then fixed and stained with Anti FGFR1(P252T) monoclonal antibody (Cat. #26253). |
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