Anti BRaf(V600E) Mouse Monoclonal Antibody

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Anti- BRaf(V600E) Mouse Monoclonal Antibody

價格	￥4800.00
品牌	NewEast Bioscienes
產地	中國武漢
貨號	26039
規格	100ul
說明書	說明書下載

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BRaf(V600E) [26039]
[100 µL]

Catalog Number: 26039

Gene Symbol: BRaf

Description: Anti BRaf(V600E) Mouse Monoclonal Antibody

Background: BRaf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers

Immunogen: A synthetic peptide from the internal region of BRaf(V600E), human origin.

Applications: ELISA, WB, IHC, IP

Recommended Dilutions:
ELISA: 1:100-1:5000
WB: 1:100-1:2000
IHC: 1:100-1:1000

Concentration: 1 mg/ml

Host: mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG2b

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes BRaf(V600E), but not wild type BRaf proteins from vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Western blot:

Western blot analysis of recombinant Braf proteins with anti-BRaf(V600E) antibody. Purified His - tagged BRaf(V600E ) ?amino acids 513-693)(lane1) ? corresponding wild type protein(lane 2), His - tagged BRaf(V600D ) (lane 3) , His - tagged BRaf(V600A ) (lane 4) , His - tagged BRaf(V600G ) (lane 5) , His - tagged BRaf(V600K ) (lane 6), His - tagged BRaf(V600R ) (lane 7), and His - tagged BRaf( V600M ) (lane 8) were blotted with anti BRaf(V600E) monoclonal antibody (Cat # 26039) .

Immunofluorescence:

Immunofluorescence of cell expressing BRaf protein with anti BRaf(V600E) antibody . HEK293T cells were transfected with pCDNA3-GFP BRaf(V600E )plasmid,pCDNA3-GFP BRaf( WT )plasmid, pCDNA3-GFP BRaf(V600K )plasmid,pCDNA3-GFP BRaf(V600R )plasmid, pCDNA3-GFP- BRaf(V600A )plasmid,pCDNA3-GFP BRaf(V600D ) plasmid, pCDNA3-GFP-BRaf(V600M)plasmid, or pCDNA3-GFP-B-Raf(V600G)plasmid, then fixed and stained with anti BRaf(V600E) monoclonal antibody (Cat. # 26039).

Immunohistochemistry:

Immunohistochemical analysis of paraffin-embedded human melanoma tissue with anti BRaf(V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:100) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1:50) was used as secondary antibody.

Immunohistochemical analysis of paraffin embedded Thyroid Carcinoma tissue -with

anti BRaf(V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with paraffin.

Samples were then incubated with primary antibody (1:300) overnight at 4°C.

Related Products
Catalog#	Name	Size	Price
21271	Anti BRaf Rabbit Polyclonal Antibody	100 μL	￥2600
26129	Anti BRaf Mouse Monoclonal Antibody	100 μL	￥2800
26109	Anti BRaf(N581S) Mouse Monoclonal Antibody	100 μL	￥4800
26039	Anti BRaf(V600E) Mouse Monoclonal Antibody	100 μL	￥4800
26110	Anti BRaf(V600K) Mouse Monoclonal Antibody	100 μL	￥4800
26184	Anti BRaf(V600D) Mouse Monoclonal Antibody	100 μL	￥4800
26182	Anti BRaf(V600R) Mouse Monoclonal Antibody	100 μL	￥4800
26196	Anti BRaf(G469E) Mouse Monoclonal Antibody	100 μL	￥4800

26199	Anti BRaf(V600A) Mouse Monoclonal Antibody	100 μL	￥4800
26462	Anti BRAF(V600M) Mouse Monoclonal Antibody	100 μL	￥4800

Publications:

1. Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma
Mod Pathol. 2013 Mar;26(3):414-20.

2. Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients
Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733

3. Prospective immunohistochemical analysis of BRAF V600E mutation in melanoma
human pathology. February 2015Volume 46, Issue 2, Pages 169–175

4. Comparison of 2 monoclonal antibodies for immunohistochemical detection of BRAF V600E mutation in malignant melanoma, pulmonary carcinoma, gastrointestinal carcinoma, thyroid carcinoma, and gliomas
human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570

5. BRAF V600E Mutation-Specific Antibody: A Review

Seminars in Diagnostic Pathology

6.Reactivation of ERK and Akt confers resistance of mutant BRAF colon cancer cells to the HSP90 inhibitor AUY922

Oncotarget. 2016 Aug 2; 7(31): 49597–49610.

7.PES1 promotes the occurrence and development of papillary thyroid cancer by upregulating the ERα/ERβ protein ratio

Sci Rep. 2019; 9: 1032.

8.Targeting of the MAPK and AKT pathways in conjunctival melanoma shows potential synergy

Oncotarget. 2017 Aug 29; 8(35): 58021–58036

9.Clinical, Histologic, and Immunophenotypic Features of Serrated Polyps in Patients With Inflammatory Bowel Disease

Gastroenterology Res. 2018 Oct; 11(5): 355–360

A Novel Approach for the Detection and Genetic Analysis of Live Melanoma Circulating Tumor Cells 2024-10-09
發表時間：2015-03-25

名稱/貨號：Anti- BRaf(V600E) Mouse Monoclonal Antibody /26039

影響因子：PLOS ONE IF=3.7

應用范圍：IF（作為臨床診斷工具的驗證 CTC）

種屬：Human (黑色素瘤）
Immunohistochemistry as a reliable method for detection of BRAF-V600E mutation in melanoma: a systematic review and meta-analysis of current published literature 2024-10-09
發表時間：2016-06-01

名稱/貨號：Anti- BRaf(V600E) Mouse Monoclonal Antibody /26039

影響因子：Journal of Surgical Research IF=2.2

應用范圍：IHC（作為臨床診斷工具的驗證與VE1 同等）

種屬：Human
Concomitant high expression of BRAFV600E, P‐cadherin and cadherin 6 is associated with High TNM stage and lymph node metastasis in conventional papillary thyroid carcinoma 2024-10-09
發表時間：2014-02-23

名稱/貨號：Anti- BRaf(V600E) Mouse Monoclonal Antibody /26039

影響因子：Clinical Endocrinology IF=3.2

應用范圍：IHC（作為臨床診斷工具的驗證）

種屬：Human（甲狀腺瘤）
Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients 2024-10-09
發表時間：2014-02-23

名稱/貨號：Anti- BRaf(V600E) Mouse Monoclonal Antibody /26039

影響因子：Tumor Biology

應用范圍：IHC（作為臨床診斷工具的驗證）

種屬：Human（黑色素瘤）
Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma 2024-10-09
發表時間：2013-03-01

名稱/貨號：Anti- BRaf(V600E) Mouse Monoclonal Antibody /26039

影響因子：Modern Pathology IF=7.5

應用范圍：IHC（作為臨床診斷工具的驗證）

種屬：Human（黑色素瘤，甲狀腺瘤，結直腸癌）

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