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Anti- Ras(G12D) Mouse Monoclonal Antibody

價格￥4800.00
品牌NewEast Biosciences   
產地中國 武漢
貨號26036
規格100ul
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  • Ras(G12D) [26036]
    [100 µL]

     

    Catalog Number:  26036
    Gene Symbol:  RAS(G12D)
    Description:   Anti Ras(G12D) Mouse Monoclonal Antibody
    Background:   Ras(G12D) is one type of Ras missense mutations at the codon 12. Ras protein is a membrane-associated GTPase that regulates cell proliferation, differentiation, and survival. Ras G12D mutation results in decreased GTPase activity and constitutive signaling. This mutation is found in many types of human cancers.
    Immunogen:    A synthetic peptide from the internal region of Ras which includes the mutation of G12D, human origin.
    Applications:    ELISA, WB, IF, IHC
    Recommended Dilutions:   
    ELISA: 1:100-1:3000 
    WB: 1:200-1:1000
    IF: 1:50-1:100
    IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG2a
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Ras(G12D) antibody recognizes Ras(G12D) proteins of vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
     
    Western blot:  
    Western blot analysis of recombinant Ras WT and G12D proteins. Equal amount of purified Ras WT (lane 1, cat. #10172) and G12D (lane 2, cat.# 10154) proteins were loaded and blotted with anti-Ras G12D mouse monoclonal antibody(Cat. # 26036) (top panel). Input control (bottom panel) was blotted with rabbit polyclonal anti-Ras antibody (Cat. # 21021）
    Immunofluorescence:  
    image.png

     

    Immunofluorescence of cells expressing RAS proteins with anti RAS(G12D) antibody. HEK293T cells were transfected with pCDNA3-GFP Ras(G12D) plasmid, pCDNA3-GFP Ras(WT)plasmid,pCDNA3-GFP Ras(G12S) plasmid, pCDNA3-GFP Ras(G12V)plasmid,pCDNA3-GFP Ras(G12C)plasmid, pCDNA3-GFP Ras(G12R)plasmid,or pCDNA3-GFP Ras(G12A)plasmid, then fixed and stained with Anti Ras(G12D) monoclonal antibody (Cat. # 26036).
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    26193 Anti Ras(Q61L) Mouse Monoclonal Antibody 100 μL ￥4,800
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    26038 Anti Ras(G13D) Mouse Monoclonal Antibody 100 μL ￥4,800
    26036 Anti Ras(G12D) Mouse Monoclonal Antibody 100 μL ￥4,800

     

     

    Publications:
    1.  DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model
        Gene Therapy 21, 888-896 (October 2014)
    2.  Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a caecal adenocarcinoma and contiguous tubulovillous adenoma
        J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136

     

     

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