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Active Gαi GTP [26901]
[30 µL]

Catalog Number:  26901

Gene Symbol:  Gnai

Description:   Anti Active Gαi Mouse Monoclonal Antibody

Background:   Heterotrimeric G proteins are critical cellular signal transducers. Gαirepresents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαi proteins are being explored.

Immunogen:   Recombinant full length protein of active Gαi1

Applications:   IP, IHC

Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP;                                                     1:100 dilution for IHC

Concentration:   1 mg/ml

Host:   mouse

Format:   Liquid

Clonality:   Monoclonal

Isotype:   IgG1

Purity:   Purified from ascites

Storage buffer:

Preservative:  no

Constituents:  PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity:  Anti active Gαi antibody recognizes active Gαi1, Gαi2, and Gαi3 of vertebrates.

Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Immunoprecipitation/Western blot:

                                             Gα_i activation assay. 
A. CHO cells were

 transfected with wild-type Gα_i1 (lanes 1 and 2) or constitutively

 active Gα_i1-Q204L (lane 3). Cell lysates were treated with GDP(lane 1) or GTPγS 

(lane 3). Lysates were then incubated with an anti-active Gα_i monoclonal antibody 

(Cat. No.26901) (top panel). The precipitated active Gα_i was immunoblotted with 

an anti Gα_i monoclonal antibody (Cat. No. 26003). The bottom panel shows the 

Western blot with anti Gα_i monoclonal antibody (Cat.No. 26003) of the cell lysates. 

B. HEK293 cells stably expressing human m2 mAChR were treated with (lane 2) or 

without (lane 1)carbachol. Cell lysates were then incubated with an anti active Gα_i monoclonal antibody 

(Cat. No. 26901) (top panel).The precipitated active Ga _i was immunoblotted with an anti Gα_i rabbit polyclonal

 antibody (Cat. No. 21006). The bottom panel shows the Western blot with anti tubulin of the cell lysates.

GI,Gai,gi