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Anti- Active Cdc42 Mouse Monoclonal Antibody

價格￥4800.00
品牌NewEast Biosciences   
產地中國 武漢
貨號26905
規格30μl
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  • Active Cdc42 GTP [26905]
    [30 µL]

     

     

    Catalog Number:  26905
    Gene Symbol:  CDC42
    Description:   Anti Active Cdc42 Mouse Monoclonal Antibody
    Background:   Small GTPases are a super-family of cellular signaling regulators. Cdc42 belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Cdc42, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).
    Immunogen:   Recombinant full length protein of active Cdc42
    Applications:   IP, IHC
    Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP; 
                                                       1:100 dilution for IHC
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG1
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti active Cdc42 antibody recognizes active Cdc42 from vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
     
    IP-Western blot:  
    IP/WB analysis of active Cdc42 protein. Purified recombinant Cdc42 proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti active Cdc42 mouse monoclonal antibody (Cat. # 26905). After SDS/PAGE, the membrane filter was probed with anti Cdc42 mouse monoclonal antibody (Cat. # 26008).
    Immunofluorescence:
    Immunofluorescent labeling of Cdc42-GTP in MS1 mouse endothelial cells. The MS1 cells cultured on glass coverslips (Fisher) in DMEM containing 5% FBS were fixed with 4% paraformaldehyde for 10 minutes at RT (room temperature) and permeablized in PBSN (PBS+0.1%NP40) for 15 minutes at RT. The cells were stained with primary antibody (Cdc42 GTP) at 1:25 dilution in Invitrogen's CAS-BLOCK (00-8120) reagent at RT for 1 hour. After brief washing in PBSN (3x5min), a secondary Alexa555 conjugated goat anti mouse antibody (Invitrogen A21424) was applied in 1:200 in CAS-BLOCK at RT for 1 hour. The coverslips were then washed in PBSN (3x5min) and mounted using vectorsheld's hard set mounting medium (H-1500), and examined using a Zeiss inverted epifluorescent miscroscope.

     

     

    Related Products
    Catalog# Name Size Price
    80701 Cdc42 pulldown  Activation Assay Kit  30 assays ￥6,800
    26905 Anti- Active Cdc42 Mouse Monoclonal Antibody  30 μL ￥4,800
    26008 Anti Cdc42 Mouse Monoclonal Antibody  100 μL ￥2800  
    21010 Anti Cdc42 Rabbit Polyclonal Antibody  100 μL  ￥2600  

     

     

     

  • Calculated molecular weight  :   21 kDa
    Observed molecular weight   :  21 kDa

    Uniprot:                                     P60953

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